Synaptotagmin-9 (SYT9) may be involved in Ca2+-dependent exocytosis of secretory vesicles through Ca2+ and phospholipid binding to the C2 domain or may serve as Ca2+ sensors in the process of vesicular trafficking and exocytosis.
Human Synaptotagmin-9 (SYT9) ELISA Kit employs a two-site sandwich ELISA to quantitate SYT9 in samples. An antibody specific for SYT9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySYT9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYT9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYT9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Synaptotagmin-9 (SYT9) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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