Stanniocalcin-1 is a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. The gene contains a 5' UTR rich in CAG trinucleotide repeats. The encoded protein contains 11 conserved cysteine residues and is phosphorylated by protein kinase C exclusively on its serine residues.The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. Overexpression of human stanniocalcin 1 in mice produces high serum phosphate levels, dwarfism, and increased metabolic rate. This gene has altered expression in hepatocellular, ovarian, and breast cancers.
Human Stanniocalcin-1 (STC1) ELISA Kit employs a two-site sandwich ELISA to quantitate STC1 in samples. An antibody specific for STC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySTC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for STC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Stanniocalcin-1 (STC1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/human-stanniocalcin-1-stc1-elisa-kit-kte60383/
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