Human Signal transducer and activator of transcription 4 (STAT4) ELISA Kit,Abbkine_specification/price/image

Product Detail
Company Profile

STAT4 is phosphorylated in response to interleukin-12 and is essential for IL12 signal transduction. For further information on STATs. STAT4 and STAT1, which both map to chromosome 2q32, may have arisen via a tandem gene duplication. However, STAT1 was expressed ubiquitously, whereas STAT4 was expressed in specific tissues, including spleen, heart, brain, peripheral blood cells, and testis. STAT4 expression was drastically increased in T cells following treatment with a DNA methyltransferase inhibitor. Truncation of methylation sites in the proximal regulatory elements of the STAT4 promoter markedly enhanced transcriptional activity. The N-terminal protein interaction domain (N domain) of STAT4 is required for STAT4 activation after IL12 signaling.

Human Signal transducer and activator of transcription 4 (STAT4) ELISA Kit employs a two-site sandwich ELISA to quantitate STAT4 in samples. An antibody specific for STAT4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySTAT4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for STAT4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STAT4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Signal transducer and activator of transcription 4 (STAT4) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/human-signal-transducer-and-activator-of-transcription-4-stat4-elisa-kit-kte60378/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.