Human Signal transducer and activator of transcription 3 (STAT3) ELISA Kit,Abbkine_specification/price/image

Product Detail
Company Profile

STAT3, a transcription factor for acute phase response genes, acts as an adaptor molecule in signal transduction from the type I interferon receptor. They found that it binds to a conserved sequence in the cytoplasmic tail of the IFNAR1 chain of the receptor and undergoes interferon-dependent tyrosine phosphorylation. The p85 regulatory subunit of phosphatidylinositol 3-kinase, which activates a series of serine kinases, was found to bind to phosphorylated STAT3 and subsequently to undergo tyrosine phosphorylation. The authors concluded that STAT3 acts as an adaptor to couple another signaling pathway to the interferon receptor.In many human cancers and transformed cell lines, STAT3 is persistently activated, and in cell culture, active STAT3 is either required for transformation, enhances transformation, or blocks apoptosis.

Human Signal transducer and activator of transcription 3 (STAT3) ELISA Kit employs a two-site sandwich ELISA to quantitate STAT3 in samples. An antibody specific for STAT3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySTAT3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for STAT3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STAT3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Signal transducer and activator of transcription 3 (STAT3) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/human-signal-transducer-and-activator-of-transcription-3-stat3-elisa-kit-kte60377/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.