Human 3-oxo-5-alpha-steroid 4-dehydrogenase 2 (SRD5A2) ELISA Kit,Abbkine_specification/price/image

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SRD5a2 encodes a microsomal protein expressed at high levels in androgen-sensitive tissues such as the prostate. The encoded protein is active at acidic pH and is sensitive to the 4-azasteroid inhibitor finasteride. Deficiencies in this gene can result in male pseudohermaphroditism, specifically pseudovaginal perineoscrotal hypospadias (PPSH).Steroid 5-alpha-reductase catalyzes the conversion of testosterone into the more potent androgen, dihydrotestosterone (DHT).The cDNA contains a long 3-prime untranslated region and encodes a hydrophobic 254-amino acid protein that shares 50% sequence identity with human SRD5A1 and 46% identity with rat 5-alpha-reductase. The biochemical and pharmacologic properties of the enzyme were consistent with its being the major isozyme in genital tissue.

Human 3-oxo-5-alpha-steroid 4-dehydrogenase 2 (SRD5A2) ELISA Kit employs a two-site sandwich ELISA to quantitate SRD5A2 in samples. An antibody specific for SRD5A2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySRD5A2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SRD5A2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SRD5A2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human 3-oxo-5-alpha-steroid 4-dehydrogenase 2 (SRD5A2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.