Human Sequestosome-1 (SQSTM1) ELISA Kit,Abbkine_specification/price/image

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The Src homology type 2 (SH2) domain is a highly conserved motif of about 100 amino acids which mediates protein-protein interactions by binding to phosphotyrosine. p56-lck, a T-cell-specific src family tyrosine kinase with an SH2 domain, is involved in T-cell signal transduction. A 62-kD protein (Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa) was identified by Park et al. (1995) as a ligand of the p56-lck SH2 domain. Gong et al. (1999) screened a yeast 2-hybrid library made from rat hippocampal mRNA against full-length Kv-beta-2 . Two previously unknown interacting proteins were identified, one of which was identical to Zip (PKC-zeta)-interacting protein), the rat homolog of p62. Zip1 and Zip2, which arise from alternative splicing, are identical except for 27 residues missing from Zip2.

Human Sequestosome-1 (SQSTM1) ELISA Kit employs a two-site sandwich ELISA to quantitate SQSTM1 in samples. An antibody specific for SQSTM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySQSTM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SQSTM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SQSTM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Sequestosome-1 (SQSTM1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/human-sequestosome-1-sqstm1-elisa-kit-kte60354/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.