Uni®Mab is a high performance Protein A affinity chromatography medium, introduced by Nano-Micro with its patented technology developed in-house. This medium is based on the monodisperse porous poly (methyl methacrylate) (PMMA) microspheres as the matrix, and a genetically modified protein A as the ligand, which ensures its high affinity binding of monoclonal antibodies and Fc fragment containing recombinant proteins (macromolecules). Uni®Mab has come with high mechanical strength and good pH stability. Even at a high flow rate, it still maintained a high dynamic binding capacity, meeting the demands from laboratory preparationto industrial production.

Characteristics and Advantages

Highly uniform particles
Uni®Mab affinity chromatography medium has highly uniform particle size distribution and precisely controlled porestructure, as shown in Fig. 1.

Figure 1. SEM diagram of Uni^®Mab and a leading brand of protein A medium

High flow rate
Uni^®Mab affinity chromatography medium was made from polymethyl methacrylate (PMMA) microspheres (as matrix); and it would withstand the pressure of 0.5 MPa. When compared to an affinity medium with convention alagarose matrix of the chromatography media, it would make the separation process much faster, thus reducing the time purification, for hundreds of liters of large scale chromatography preparative column.(Experimental：Column：4.6 mm × 50 mm   Mobile phase：50 mM PBS，0.5 M NaCl)

Figure 2. Comparison of Uni®Mab and a conventional agarose type protein A medium, their pressure vs. linear flow rate

Excellent loading capacity
At a high flow rate, Uni^®Mab showed a significantly better dynamic binding capacity (DBC) than that of the conventional agarose type protein A medium.  The same observation was also valid, even when the residence time was increase to 4 - 6 minute. (Experimental：Column：7 mm × 25 mm  Sample：Human IgG，2 mg/mL Equilibration buffer：20 mM PBS，pH 7.0 ，0.15 M NaCl   Elution：0.1 M Glycine, pH 3.0)

Figure 3. Comparison of Uni®Mab with a leading brand of Protein A medium, for their dynamic binding capacity

Outstanding pH stability
Uni^®Mab affinity chromatography medium could be applied in a pH range from 3 - 12, and it could be cleaned with 0.1 - 0.5 M NaOH. In a life-cycle test, Uni^®Mab medium remained greater than 90% of the original dynamic binding capacity (DBC), after 100 CIP cycles with 0.5 M NaOH as CIP reagent. (CIP procedure:Medium:Uni^®Mab Column：7 mm × 25 mm   Sample:recombinant antibody(chimera) DBC calculation:10% breakthrough Equilibration buffer：20 mM PBS, 150 mM NaCl, pH 7.2, 3 CV（column volume)   Elution：20 mM Citrate, pH 3.0  CIP:0.5 M NaOH, 15 CV  Re-equilibration:10 CV)

Figure 4. Uni®Mab pH stability in life cycle tests

Application

Uni^®Mab was secessfully applied in purification of hIgG from human serum. (Experimental：Column：7 mm× 25 mm  Sample：human serum, 5 mL  Equilibration Buffer：20mM PBS, pH 7.0  0.15 M NaCl   Elution：0.1 M Glycine, pH 3.0  Residence time：4 min)

Figure 5.Uni^®Mab used in hIgG purification from human serum

Technical information