Transportin-2 (KPNB2) interacts directly and specifically with M9, the bidirectional transport signal of the nuclear shuttling protein hnRNPA1 and mediates hnRNPA1 nuclear import. In the course of isolating additional transportin-1 cDNAs, Siomi et al. (1997) isolated cDNAs encoding a related protein that they designated 'transportin-2.' The sequence of the predicted 894-amino acid protein shares 84% identity with that of transportin-1. One notable difference is that transportin-2 contains a short extra sequence within the region corresponding to the M9-interacting domain of transportin-1. Far Western blotting showed that transportin-2 and transportin-1 have different substrate specificities. Siomi et al. (1997) suggested that the insert in transportin-2 modifies its interaction with import substrates.
Human Transportin-2 (TNPO2) ELISA Kit employs a two-site sandwich ELISA to quantitate TNPO2 in samples. An antibody specific for TNPO2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNPO2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNPO2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNPO2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Transportin-2 (TNPO2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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