CELF proteins, including CELF3, bound RNA containing muscle-specific splicing enhancer (MSE) elements and could activate MSE-dependent exon inclusion of cardiac troponin T (TNNT2) minigenes in transfected quail fibroblasts.The deduced 462-amino acid protein contains 2 N-terminal RNA recognition motifs (RRMs), a C-terminal RRM, several potential phosphorylation sites, and a nuclear localization signal near the C terminus. activated inclusion of exon 10 in a tau (MAPT) minigene. Deletion and mutation analyses showed that the activity of TNRC4 on tau exon 10 splicing required RRM2 in TNRC4 and an intronic element in tau pre-mRNA. The polyglutamine sequence of TNRC4 had no effect on tau exon 10 splicing. Tnrc4 -/- mice exhibited decreased inclusion of exon 10 in tau mRNA.
Human CUGBP Elav-like family member 3 (TNRC4) ELISA Kit employs a two-site sandwich ELISA to quantitate TNRC4 in samples. An antibody specific for TNRC4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNRC4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNRC4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNRC4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human CUGBP Elav-like family member 3 (TNRC4) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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