TP63 also known as the p63 gene was discovered 20 years after the the discovery of the p53 tumor suppressor gene and along with p73 constitutes the p53 gene family based on their structural similarity.Tumor protein p63 is a member of the p53 family of transcription factors. p63 mice have several developmental defects which include the lack of limbs and other tissues, such as teeth and mammary glands, which develop as a result of interactions between mesenchyme and epithelium.p63 immunostaining has utility for differentiating prostatic adenocarcinoma (the most common type of prostate cancer) and benign prostatic tissue; normal prostatic glands stain with p63 (as they have basal cells), while the malignant glands in prostatic adenocarcinoma (which lacks these cells) do not.
Human Tumor protein 63 (TP63) ELISA Kit employs a two-site sandwich ELISA to quantitate TP63 in samples. An antibody specific for TP63 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTP63 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TP63 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TP63 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Tumor protein 63 (TP63) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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