TRA2-beta gene contains 10 exons and spans 21 kb. RT-PCR and analysis of TRA2-beta cDNAs in the EST database indicated that 5 different TRA2-beta RNA isoforms are generated by alternative splicing.TRA2-beta-1 is a nuclear protein that colocalizes with SC35 in a speckled pattern. It interacts with several SR proteins in yeast. A second isoform, named TRA2-beta-2, is generated by alternative splicing. This isoform gives rise to a truncated protein without an SR domain. Both isoforms are evenly distributed throughout adult rat tissue. The ratio of these 2 isoforms changes after stimulation of primary human T-cell and primary rat spleen cell cultures, indicating that alternative splicing is involved in the regulation of TRA2-beta activity.
Human Transformer-2 protein homolog beta (TRA2B) ELISA Kit employs a two-site sandwich ELISA to quantitate TRA2B in samples. An antibody specific for TRA2B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTRA2B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRA2B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRA2B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Transformer-2 protein homolog beta (TRA2B) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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