Tumor necrosis factor (TNF) receptor-associated factors, such as TRAF7, are signal transducers for members of the TNF receptor superfamily. Fluorescence microscopy demonstrated TRAF7 localization in a vesicular pattern in the cytosol in the presence or absence of MEKK3. SDS-PAGE analysis suggested that MEKK3 phosphorylates sites in the N-terminal region of TRAF7 and induces ubiquitination. In the presence of E1 (UBE1) and E2 activating and conjugating enzymes, a TRAF7 fragment containing the RING domain was ubiquitinated, indicating that TRAF7 may have E3 ubiquitin ligase activity. Luciferase reporter and RNAi analysis established that coexpression of wildtype TRAF7 and MEKK3 resulted in a synergistic activation of NFKB and AP1.
Human Tumor necrosis factor receptor-associated factor 6 (TRAF6) ELISA Kit employs a two-site sandwich ELISA to quantitate TRAF6 in samples. An antibody specific for TRAF6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTRAF6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRAF6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRAF6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Tumor necrosis factor receptor-associated factor 6 (TRAF6) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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