Human cytomegalovirus (CMV) frequently infects young people and persists for the lifetime of the individual in a latent state, due in part to downregulation of major histocompatibility (MHC) class I expression and evasion of T cell-mediated immunity, with occasional asymptomatic episodes of viral shedding.Instead of a transmembrane domain, ULBP3 has a putative glycosylphosphatidylinositol (GPI) linkage to the membrane. Enzymatic analysis confirmed that the ULBPs are indeed GPI-linked at the cell surface. RT-PCR analysis detected ULBP expression in T, B, and erythroleukemia cell lines and in a wide range of tissues. Expression was upregulated in colon and stomach tumors and downregulated in kidney tumors. FACS analysis demonstrated that protein expression generally correlated with message expression.
Human NKG2D ligand 2 (ULBP2) ELISA Kit employs a two-site sandwich ELISA to quantitate ULBP2 in samples. An antibody specific for ULBP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyULBP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ULBP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ULBP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human NKG2D ligand 2 (ULBP2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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