Synaptic vesicles are responsible for regulating the storage and release of neurotransmitters in the nerve terminal. Northern blot analysis revealed that a 5.8-kb transcript is expressed in electromotor neurons. Western blot analysis determined expression of a 42-kD protein in the electric organ that copurified with synaptic vesicles.Smith et al. (1996) identified the sequence of 2 complete genes within 117 kb of DNA containing the BRCA1 gene: RHO7 and VAT1, an abundant membrane protein of cholinergic synaptic vesicles. The coding sequence of VAT1 predicts a 301-amino acid peptide. The authors found that a CpG island precedes the VAT1 gene, which contains 6 exons spanning 8.1 kb. They determined the following order of genes in this region: cen--IFP35--VAT1--RHO7--BRCA1--1A1-3B--tel.
Human Vasodilator-stimulated phosphoprotein (VASP) ELISA Kit employs a two-site sandwich ELISA to quantitate VASP in samples. An antibody specific for VASP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVASP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VASP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VASP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Vasodilator-stimulated phosphoprotein (VASP) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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