VEGF-A is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized.VEGF is a chemical signal produced by cells that stimulates the growth of new blood vessels. The most important member is VEGF-A. Other members are Placenta growth factor (PlGF), VEGF-B, VEGF-C and VEGF-D. The latter ones were discovered later than VEGF-A, and, before their discovery, VEGF-A was called just VEGF.
Human Vascular endothelial cell growth factor (VEGF165) ELISA Kit employs a two-site sandwich ELISA to quantitate VEGF165 in samples. An antibody specific for VEGF165 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVEGF165 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VEGF165 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VEGF165 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Vascular endothelial cell growth factor (VEGF165) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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