ISG15 shares several common properties with other ubiquitin-like molecules (UBLs), but its activity is tightly regulated by specific signaling pathways that have a role in innate immunity. ISG16 was identified as an interferon stimulated gene (ISG) since̞Ⴠ̞ၰ̞
Goat Ubiquitin-like protein ISG15 (ISG15) ELISA Kit employs a two-site sandwich ELISA to quantitate ISG15 in samples. An antibody specific for ISG15 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyISG15 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ISG15 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ISG15 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Goat Ubiquitin-like protein ISG15 (ISG15) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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