LPS is an endotoxin, and induces a strong response from normal animal immune systems.LPS acts as the prototypical endotoxin because it binds the CD14/TLR4/MD2 receptor complex, which promotes the secretion of pro-inflammatory cytokines in many cell types, but especially in macrophages. In Immunology, the term "LPS challenge" refers to the process of exposing a subject to an LPS which may act as a toxin.
Goat Lipopolysaccharides (LPS) ELISA Kit employs a two-site sandwich ELISA to quantitate LPS in samples. An antibody specific for LPS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLPS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LPS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LPS bound in the initial step. The color development is stopped and the intensity of the color is measured.
Goat Lipopolysaccharides (LPS) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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