Macrophage Colony-Stimulating Factor (M-CSF) is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. M-CSF has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13.
Goat Macrophage Colony-Stimulating Factor (M-CSF) ELISA Kit employs a two-site sandwich ELISA to quantitate MCSF in samples. An antibody specific for MCSF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMCSF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MCSF is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MCSF bound in the initial step. The color development is stopped and the intensity of the color is measured.
Goat Macrophage Colony-Stimulating Factor (M-CSF) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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