AST) is similar to alanine transaminase (ALT) in that it is another enzyme associated with liver parenchymal cells.It is raised in acute liver damage. It is also present in red blood cells and cardiac muscle, skeletal muscle, and kidney and brain tissue, and may be elevated due to damage to those sources as well.AST was defined as a biochemical marker for the diagnosis of acute myocardial infarction in 1954. However the use of AST for such a diagnosis is now redundant and has been superseded by the cardiac troponins.The 2 isoenzymes are homodimeric. In liver about 80% of the enzyme activity is mitochondrial in origin, whereas in serum the enzyme activity is largely cytosolic.
Fish Aspartate aminotransferase (AST) ELISA Kit employs a two-site sandwich ELISA to quantitate GOT1 in samples. An antibody specific for GOT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGOT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GOT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GOT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Fish Aspartate aminotransferase (AST) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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