17-Hydroxypregnenolone is a 21-carbon steroid that is converted from PREGNENOLONE by STEROID 17-ALPHA-HYDROXYLASE. It is an intermediate in the delta-5 pathway of biosynthesis of GONADAL STEROID HORMONES and the adrenal CORTICOSTEROIDS.
Fish 17-hydroxypregnenolone ELISA Kit employs a two-site sandwich ELISA to quantitate 17-hydroxypregnenolone in samples. An antibody specific for 17-hydroxypregnenolone has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any17-hydroxypregnenolone present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for 17-hydroxypregnenolone is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of 17-hydroxypregnenolone bound in the initial step. The color development is stopped and the intensity of the color is measured.
Fish 17-hydroxypregnenolone ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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