Ovalbumin is the main protein found in egg white, making up 60-65% of the total protein. While Ovalbumin displays sequence and three-dimensional homology to the serpin superfamily, it is a noninhibitory serpin . While most serpins control such processes as fibrinolysis and coagulation by inhibiting serine proteases, the function of ovalbumin is unknown, although it is presumed to be a storage protein.The ovalbumin protein of chickens is made up of 385 amino acids, and its relative molecular mass is 45 kDa. It is a glycoprotein with 4 sites of glycosylation.It is secreted from the cell. Unlike the signal sequences of most secreted proteins, Ovalbumin's signal sequence is not cleaved. Additionally it is not located directly on the N-terminus, but rather internally, starting at residue 21 and ending at residue 47.
Chicken Ovalbumin (OVA) ELISA Kit employs a two-site sandwich ELISA to quantitate OVA in samples. An antibody specific for OVA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyOVA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for OVA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of OVA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Chicken Ovalbumin (OVA) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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