Taste receptor type 1 member 3 (TAS1R3) is a G-protein coupled receptor involved in taste responses. TAS1R3 can form a heterodimeric receptor with TAS1R1 to elicit the umami taste response, or it can bind with TAS1R2 to form a receptor for the sweet taste response.
Canine Taste receptor type 1 member 3 (TAS1R3) ELISA Kit employs a two-site sandwich ELISA to quantitate TAS1R3 in samples. An antibody specific for TAS1R3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTAS1R3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAS1R3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAS1R3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Canine Taste receptor type 1 member 3 (TAS1R3) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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