TAPBP encodes a transmembrane glycoprotein which mediates interaction between newly assembled major histocompatibility complex (MHC) class I molecules and the transporter associated with antigen processing (TAP), which is required for the transport of antigenic peptides across the endoplasmic reticulum membrane. This interaction is essential for optimal peptide loading on the MHC class I molecule. Up to four complexes of MHC class I and TAPBP may be bound to a single TAP molecule. Tapasin contains a C-terminal double-lysine motif (KKKAE) known to maintain membrane proteins in the endoplasmic reticulum. This gene lies within the major histocompatibility complex on chromosome 6. Alternative splicing results in three transcript variants encoding different isoforms.
Canine Tapasin (TAPBP) ELISA Kit employs a two-site sandwich ELISA to quantitate TAPBP in samples. An antibody specific for TAPBP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTAPBP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAPBP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAPBP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Canine Tapasin (TAPBP) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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