beta-lactoglobulin is the major whey protein of cow's milk and is also present in many other mammalian species. Its structure, properties and biological role have been reviewed many times.Unlike the other main whey protein, alpha-lactalbumin, no clear function has been identified for β-lactoglobulin, although it binds to several hydrophobic molecules, suggesting its role in their transport. The strong suggestion is that the molecule exists primarily as a food source. Several genetic variants have been identified, the main ones in the cow being labelled A and B. Because of its abundance and ease of purification, it has been subjected to a wide range of biophysical studies. Its structure has been determined several times by X-ray crystallography and NMR. beta-lactoglobulin is of direct interest to the food industry since its properties can variously be advantageous or disadvantageous in dairy products and processing .
Bovine Beta-lactoglobulin (Beta-LG) (allergen Bos d 5) (LGB) ELISA Kit employs a two-site sandwich ELISA to quantitate LGB in samples. An antibody specific for LGB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLGB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LGB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LGB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine Beta-lactoglobulin (Beta-LG) (allergen Bos d 5) (LGB) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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