Protein maestro (MRO) is specifically transcribed in males before and after differentiation of testis, and the MRO may play an important role in a mammalian sex determination. Multiple transcript variants encoding different isoforms have been found for MRO.
Bovine Protein maestro (MRO) ELISA Kit employs a two-site sandwich ELISA to quantitate MRO in samples. An antibody specific for MRO has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMRO present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MRO is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MRO bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine Protein maestro (MRO) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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