Myotrophin (MTPN) is bi-cistronic and can encode both myotrophin and leucine zipper protein 6. The myotrophin protein is associated with cardiac hypertrophy, where it is involved in the conversion of NFkappa B p50-p65 heterodimers to p50-p50 and p65-p65 homodimers. MTPN also has a potential function in cerebellar morphogenesis, and it may be involved in the differentiation of cerebellar neurons, particularly of granule cells. A cryptic ORF at the 3' end of this transcript uses a novel internal ribosome entry site and a non-AUG translation initiation codon to produce leucine zipper protein 6, a 6.4 kDa tumor antigen that is associated with myeloproliferative disease.
Bovine Myotrophin (MTPN) ELISA Kit employs a two-site sandwich ELISA to quantitate MTPN in samples. An antibody specific for MTPN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMTPN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MTPN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MTPN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine Myotrophin (MTPN) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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