MTP encodes the large subunit of the heterodimeric microsomal triglyceride transfer protein. Protein disulfide isomerase (PDI) completes the heterodimeric microsomal triglyceride transfer protein, which has been shown to play a central role in lipoprotein assembly. Mutations in MTP can cause abetalipoproteinemia.
Bovine Microsomal triglyceride transfer protein large subunit (MTTP) ELISA Kit employs a two-site sandwich ELISA to quantitate MTTP in samples. An antibody specific for MTTP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMTTP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MTTP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MTTP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine Microsomal triglyceride transfer protein large subunit (MTTP) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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