Myogenic factor 6 (MYF6) is a probable basic helix-loop-helix (bHLH) DNA binding protein involved in muscle differentiation. MYF6 likely acts as a heterodimer with another bHLH protein. Defects in MYF6 are a cause of autosomal dominant centronuclear myopathy (ADCNM).
Bovine Myogenic factor 6 (MYF6) ELISA Kit employs a two-site sandwich ELISA to quantitate MYF6 in samples. An antibody specific for MYF6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYF6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYF6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYF6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine Myogenic factor 6 (MYF6) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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