MYL3 encodes myosin light chain 3, an alkali light chain also referred to in the literature as both the ventricular isoform and the slow skeletal muscle isoform. Mutations in MYL3 have been identified as a cause of mid-left ventricular chamber type hypertrophic cardiomyopathy.
Bovine Myosin light chain 3 (MYL3) ELISA Kit employs a two-site sandwich ELISA to quantitate MYL3 in samples. An antibody specific for MYL3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYL3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYL3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYL3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine Myosin light chain 3 (MYL3) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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