Myosin, a structural component of muscle, consists of two heavy chains and four light chains. Myosin regulatory light polypeptide 9 (MYL9) is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. MYL9 binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for MYL9.
Bovine Myosin regulatory light polypeptide 9 (MYL9) ELISA Kit employs a two-site sandwich ELISA to quantitate MYL9 in samples. An antibody specific for MYL9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYL9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYL9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYL9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine Myosin regulatory light polypeptide 9 (MYL9) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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