Myosin-Ic (MYO1C) encodes a member of the unconventional myosin protein family, which are actin-based molecular motors. MYO1C is found in the cytoplasm, and one isoform with a unique N-terminus is also found in the nucleus. The nuclear isoform associates with RNA polymerase I and II and functions in transcription initiation. The mouse ortholog of this protein also functions in intracellular vesicle transport to the plasma membrane. Multiple transcript variants encoding different isoforms have been found for this gene. The related gene myosin IE has been referred to as myosin IC in the literature, but it is a distinct locus on chromosome 19.
Bovine Myosin-Ic (MYO1C) ELISA Kit employs a two-site sandwich ELISA to quantitate MYO1C in samples. An antibody specific for MYO1C has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYO1C present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYO1C is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYO1C bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine Myosin-Ic (MYO1C) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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