N6AMT2, Belongs to the methyltransferase superfamily. AML1 family. N6AMT2is a 214 amino acid protein that is encoded by a gene located on human chromosome 13q12.11. A methyltransferase is a type of transferase enzyme that transfers a methyl group from a donor to an acceptor. Methytransferases use a reactive methyl group bound to sulfur in S-adenosyl methionine (SAM) as the methyl donor. DNA methylation is often utilized to silence and regulate genes without changing the original DNA sequence, an example of epigenetic modification. This methylation occurs on cytosine residues. DNA methylation may be necessary for normal growth from embryonic stages in mammals. When mutant embryonic stem cells lacking the murine DNA methyltransferase gene were introduced to a germline of mice, they caused a recessive lethal phenotype.
Bovine N (6)-adenine-specific DNA methyltransferase 2 (N6AMT2) ELISA Kit employs a two-site sandwich ELISA to quantitate N6AMT2 in samples. An antibody specific for N6AMT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyN6AMT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for N6AMT2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of N6AMT2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine N (6)-adenine-specific DNA methyltransferase 2 (N6AMT2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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