Nuclear apoptosis-inducing factor 1 (NAIF1) was previously reported to induce apoptosis. Moreover, the expression of NAIF1 was significantly down-regulated in human gastric cancer tissues compared to adjacent normal tissues. However, the mechanism by which the NAIF1 gene induces apoptosis is not fully understood.
Bovine Nuclear apoptosis-inducing factor 1 (NAIF1) ELISA Kit employs a two-site sandwich ELISA to quantitate NAIF1 in samples. An antibody specific for NAIF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNAIF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NAIF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NAIF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine Nuclear apoptosis-inducing factor 1 (NAIF1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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