Arylamine N-acetyltransferase 1 (NAT1) is one of two arylamine N-acetyltransferase (NAT) genes in the human genome, and is orthologous to the mouse and rat Nat2 genes. The enzyme encoded by NAT1 catalyzes the transfer of an acetyl group from acetyl-CoA to various arylamine and hydrazine substrates. NAT1 helps metabolize drugs and other xenobiotics, and functions in folate catabolism. Multiple transcript variants encoding different isoforms have been found for NAT1.
Bovine Arylamine N-acetyltransferase 1 (NAT1) ELISA Kit employs a two-site sandwich ELISA to quantitate NAT1 in samples. An antibody specific for NAT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNAT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NAT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NAT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine Arylamine N-acetyltransferase 1 (NAT1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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