Pregnancy-specific protein B (PSPB) has been isolated from placental tissue of cows. Antisera were developed against PSPB and by immunohistochemical techniques the protein was localized to the binucleated cells of the trophoblastic ectoderm. A radioimmunoassay (RIA) was also developed and used to detect PSPB in sera of pregnant animals. The RIA has been used successfully in pregnancy testing in cattle and other ruminants. The assay can also be used to detect time of embryonic death. Chemical characterization of PSPB showed that it was an acidic glycoprotein with an apparent molecular weight of 78,000. It has several isoelectric variants with pIs of 4.0-4.4.
Bovine Pregnancy Specific Protein B (PSPB) ELISA Kit employs a two-site sandwich ELISA to quantitate PAG1 in samples. An antibody specific for PAG1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPAG1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PAG1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PAG1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine Pregnancy Specific Protein B (PSPB) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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