Serum paraoxonase/arylesterase 2 is a member of the paraoxonase gene family, which includes three known members located adjacent to each other on the long arm of chromosome 7. The encoded protein is ubiquitously expressed in human tissues, membrane-bound, and may act as a cellular antioxidant, protecting cells from oxidative stress.Hydrolytic activity against acylhomoserine lactones, important bacterial quorum-sensing mediators, suggests the encoded protein may also play a role in defense responses to pathogenic bacteria. Mutations in this gene may be associated with vascular disease and a number of quantitative phenotypes related to diabetes. Alternatively spliced transcript variants encoding different isoforms have been described.
Bovine Serum paraoxonase/arylesterase 2 (PON2) ELISA Kit employs a two-site sandwich ELISA to quantitate PON2 in samples. An antibody specific for PON2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPON2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PON2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PON2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine Serum paraoxonase/arylesterase 2 (PON2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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