PPM1B is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase has been shown to dephosphorylate cyclin-dependent kinases (CDKs), and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to cause cell-growth arrest or cell death. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional transcript variants have been described, but currently do not represent full-length sequences.The deduced 479-amino acid protein shows high sequence similarity to other mammalian PP2C-beta isoforms but has an extended and unique C-terminal portion.
Bovine Protein phosphatase 1B (PPM1B) ELISA Kit employs a two-site sandwich ELISA to quantitate PPM1B in samples. An antibody specific for PPM1B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPPM1B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPM1B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPM1B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine Protein phosphatase 1B (PPM1B) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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