Proline dehydrogenase 1, mitochondrial (PRODH) encodes a mitochondrial protein that catalyzes the first step in proline degradation. Mutations in PRODH are associated with hyperprolinemia type 1 and susceptibility to schizophrenia 4 (SCZD4). PRODH is located on chromosome 22q11.21, a region which has also been associated with the contiguous gene deletion syndromes, DiGeorge and CATCH22. Alternatively spliced transcript variants encoding different isoforms have been found for PRODH.
Bovine Proline dehydrogenase 1, mitochondrial (PRODH) ELISA Kit employs a two-site sandwich ELISA to quantitate PRODH in samples. An antibody specific for PRODH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRODH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRODH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRODH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine Proline dehydrogenase 1, mitochondrial (PRODH) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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