Proteolytic degradation of extracellular matrix components has been suggested to play an essential role for the occurrence of ovulation. The plasminogen activator and matrix metalloproteinase systems, which were previously believed to be crucial for ovulation, are not required in this process.PRSS35, which was upregulated by gonadotropins. PRSS23 was highly expressed in atretic follicles and it was expressed in the ovarian stroma and theca tissues just prior to ovulation. PRSS35 was expressed in the theca layers of developing follicles. It was also highly induced in granulosa cells of preovulatory follicles. PRSS35 was also expressed in the forming and regressing CL. PRSS35 may be involved in ovulation and CL formation and regression, and that PRSS23 may play a role in follicular atresia.
Bovine Inactive serine protease 35 (PRSS35) ELISA Kit employs a two-site sandwich ELISA to quantitate PRSS35 in samples. An antibody specific for PRSS35 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRSS35 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRSS35 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRSS35 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine Inactive serine protease 35 (PRSS35) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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