Resistin, a novel adipose-derived protein, has been proposed to cause insulin-resistant states in obesity. Resistin is produced by white and brown adipose tissues but has also has been identified in several other tissues, including the hypothalamus, pituitary and adrenal glands, pancreas, gastrointestinal tract, myocytes, spleen, white blood cells and plasma. Resistin antagonizes insulin action, and is down regulated by rosiglitazone and peroxisome proliferator-activated receptor gamma agonists.Resistin is elevated in patients with type 2 diabetes and may play a role in the vascular complications of this disorder. Recently, resistin has been discussed controversially as a missing link between obesity and insulin resistance.
Bovine Resistin (RETN) ELISA Kit employs a two-site sandwich ELISA to quantitate RETN in samples. An antibody specific for RETN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRETN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RETN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RETN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine Resistin (RETN) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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