A1is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. This protein may function in stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of protein kinase C-mediated phosphorylation.Reduced expression of this protein has been implicated in cardiomyopathies. S100A1 overexpression enhances cardiac contractile performance which suggests that S100A1 is a regulator of myocardial contractility. S100A1 improves cardiac performance both by regulating calcium ion handling by the sarcoplasmic reticulum and the responsiveness of myofibrils to calcium ion.
Bovine Protein S100-A1 (S100A1) ELISA Kit employs a two-site sandwich ELISA to quantitate S100A1 in samples. An antibody specific for S100A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyS100A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S100A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S100A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine Protein S100-A1 (S100A1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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