Sclerostin is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Loss-of-function mutations in this gene are associated with an autosomal-recessive disorder, sclerosteosis, which causes progressive bone overgrowth. A deletion downstream of sclerostin, which causes reduced sclerostin expression, is associated with a milder form of the disorder called van Buchem disease.
Bovine Sclerostin (SOST) ELISA Kit employs a two-site sandwich ELISA to quantitate SOST in samples. An antibody specific for SOST has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySOST present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SOST is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SOST bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine Sclerostin (SOST) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
bio-equip.cn
中文
