Spermatogenesis-associated protein 22 (Spata22) is required early in meiotic prophase in both male and female germ cells. It is necessary to achieve normal synapsis and for repair of meiotic DNA double-strand breaks.
Bovine Spermatogenesis-associated protein 22 (SPATA22) ELISA Kit employs a two-site sandwich ELISA to quantitate SPATA22 in samples. An antibody specific for SPATA22 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPATA22 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPATA22 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPATA22 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine Spermatogenesis-associated protein 22 (SPATA22) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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