Transgelin (TAGLN) is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of Transgelin (TAGLN) is unclear. Two transcript variants encoding the same protein have been found for Transgelin (TAGLN).
Bovine Transgelin (TAGLN) ELISA Kit employs a two-site sandwich ELISA to quantitate TAGLN in samples. An antibody specific for TAGLN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTAGLN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAGLN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAGLN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine Transgelin (TAGLN) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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