Synaptotagmin 1 (or synaptotagmin) is a Ca2+ sensor in the membrane of the pre-synaptic axon terminal, coded by gene SYT1 (previously SYT). Synaptotagmins (SYTs) constitute a family of membrane-trafficking proteins that are characterized by an N-terminal transmembrane region (TMR), a variable linker, and two C-terminal C2 domains - C2A and C2B. There are 15 members in the mammalian synaptotagmin family. There are several C2-domain containing protein families that are related to synaptotagmins, including transmembrane (Ferlins, E-Syts, and MCTPs) and soluble (RIMs, Munc13s, synaptotagmin-related proteins and B/K) proteins.
Bovine Synaptotagmin-1 (SYT1) ELISA Kit employs a two-site sandwich ELISA to quantitate SYT1 in samples. An antibody specific for SYT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySYT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine Synaptotagmin-1 (SYT1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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