SYNGR3 encodes an integral membrane protein. The gene belongs to the synaptogyrin gene family. Like other members of the family the protein contains four transmembrane regions. The exact function of this protein is unclear.By searching an EST database for sequences related to rat and human SYNGR1, Kedra et al. (1998) identified cDNAs encoding SYNGR2 and SYNGR3. Like other members of the synaptogyrin family, the predicted 206-amino acid SYNGR3 protein contains 4 transmembrane regions. Northern blot analysis revealed that the 2.2-kb SYNGR3 mRNA is expressed in brain and placenta only.By fluorescence in situ hybridization, Kedra et al. (1998) mapped the SYNGR3 gene to chromosome 16pter.
Bovine Synaptogyrin-3 (SYNGR3) ELISA Kit employs a two-site sandwich ELISA to quantitate SYNGR3 in samples. An antibody specific for SYNGR3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySYNGR3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYNGR3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYNGR3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine Synaptogyrin-3 (SYNGR3) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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