Anovel nuclear protein mainly expressed in S and G2 phase cells was characterized using autoantibodies from a cancer patient. cDNA clones were isolated by immunoscreening, and sequence analysis revealed that the cDNA clones encoded a 713-amino acid residue polypeptide which is provisionally named SG2NA. In the carboxyl terminal half of SG2NA, there are 6 copies of WD-40 motifs characteristic of a large family of proteins of which the prototype is beta-transducin/enhancer of split (TLE). In addition, there are nuclear localization sequence and phosphorylation sites related to the TLE proteins. Autoantibodies occurring spontaneously in certain cancer patients have been useful reagents for identifying cellular proteins and this is the first report of a novel WD-40 protein which is the target of an autoimmune response.
Bovine Striatin-3 (STRN3) ELISA Kit employs a two-site sandwich ELISA to quantitate STRN3 in samples. An antibody specific for STRN3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySTRN3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for STRN3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STRN3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine Striatin-3 (STRN3) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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