TMEM168 is a transmembrane protein (TP) protein that goes from one side of a membrane through to the other side of the membrane. Many TPs function as gateways or "loading docks" to deny or permit the transport of specific substances across the biological membrane, to get into the cell, or out of the cell as in the case of waste byproducts. As a response to the shape of certain molecules these "freight handling" TPs may have special ways of folding up or bending that will move a substance through the biological membrane. A transmembrane protein is a polytopic protein that spans an entire biological membrane. Transmembrane proteins aggregate and precipitate in water. They require detergents or nonpolar solvents for extraction, although some of them (beta-barrels) can be also extracted using denaturing agents.
Bovine Transmembrane protein 168 (TMEM168) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM168 in samples. An antibody specific for TMEM168 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM168 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM168 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM168 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine Transmembrane protein 168 (TMEM168) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
bio-equip.cn
中文
