Ubiquitously expressed in cancer cell lines.Cloning and characterization of a cDNA fragment coding beta-casein-like protein preferentially expressed in cervical adenocarcinoma cell line CAC-1.One of eight new monoclonal antibodies generated, 12G2, specifically reacted not only with beta-casein, but also with human cervical adenocarcinoma cell line CAC-1. To identify the gene encoding the putative protein (BCLP) defined by monoclonal antibody (mAb) 12G2, a complementary DNA library was constructed. the inserted cDNA fragment that showed high homology to reported DNA sequences of unknown function in normal human placenta. BCLP was overexpressed or changed its structure by carcinogenesis.
Bovine Transmembrane protein 54 (TMEM54) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM54 in samples. An antibody specific for TMEM54 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM54 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM54 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM54 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine Transmembrane protein 54 (TMEM54) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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