TMEM57 has 5 domains, including an N-terminal LAG1 domain and a central ERM-myosin filament domain. The LAG1 domain contains 3 putative transmembrane domains. In situ hybridization of mouse embryos showed that Tmem57 was brain specific.It was primarily associated with postmigratory neurons and less so with neural proliferating cells, and Tmem57 expression was progressively downregulated during brain maturation. Fluorescence-tagged Tmem57 outlined the nuclear membrane when expressed in nonneuronal cells. In primary hippocampal neurons, Tmem57 was found in neurites and their terminals, and it partially colocalized with synapsin-1 (SYN1), a presynaptic marker.
Bovine Macoilin (TMEM57) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM57 in samples. An antibody specific for TMEM57 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM57 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM57 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM57 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine Macoilin (TMEM57) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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